AustralieFrV1, Main, Exploration, bibRecord, 001750

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)

Identifieur interne : 001750 ( Main/Exploration ); précédent : 001749; suivant : 001751

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)

Auteurs : Cristian Koepfli [Australie] ; Wang Nguitragool [Thaïlande] ; Natalie E. Hofmann [Suisse] ; Leanne J. Robinson [Australie, Papouasie-Nouvelle-Guinée] ; Maria Ome-Kaius [Papouasie-Nouvelle-Guinée] ; Jetsumon Sattabongkot [Thaïlande] ; Ingrid Felger [Suisse] ; Ivo Mueller [Australie, France]

Source :

Scientific Reports [ 2045-2322 ] ; 2016.

RBID : PMC:5159915

Abstract

Accurate quantification of parasite density in the human host is essential for understanding the biology and pathology of malaria. Semi-quantitative molecular methods are widely applied, but the need for an external standard curve makes it difficult to compare parasite density estimates across studies. Droplet digital PCR (ddPCR) allows direct quantification without the need for a standard curve. ddPCR was used to diagnose and quantify P. falciparum and P. vivax in clinical patients as well as in asymptomatic samples. ddPCR yielded highly reproducible measurements across the range of parasite densities observed in humans, and showed higher sensitivity than qPCR to diagnose P. falciparum, and equal sensitivity for P. vivax. Correspondence in quantification was very high (>0.95) between qPCR and ddPCR. Quantification between technical replicates by ddPCR differed 1.5–1.7-fold, compared to 2.4–6.2-fold by qPCR. ddPCR facilitates parasite quantification for studies where absolute densities are required, and will increase comparability of results reported from different laboratories.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159915

DOI: 10.1038/srep39183
PubMed: 27982132
PubMed Central: 5159915

Affiliations:

Australie, France, Papouasie-Nouvelle-Guinée, Suisse, Thaïlande

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)</title>
<author><name sortKey="Koepfli, Cristian" sort="Koepfli, Cristian" uniqKey="Koepfli C" first="Cristian" last="Koepfli">Cristian Koepfli</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Walter & Eliza Hall Institute</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Medical Biology, University of Melbourne</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nguitragool, Wang" sort="Nguitragool, Wang" uniqKey="Nguitragool W" first="Wang" last="Nguitragool">Wang Nguitragool</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>Faculty of Tropical Medicine, Mahidol University</institution>
, Bangkok,<country>Thailand</country>
</nlm:aff>
<country xml:lang="fr">Thaïlande</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hofmann, Natalie E" sort="Hofmann, Natalie E" uniqKey="Hofmann N" first="Natalie E." last="Hofmann">Natalie E. Hofmann</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Swiss Tropical and Public Health Institute</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a5"><institution>University of Basel</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Robinson, Leanne J" sort="Robinson, Leanne J" uniqKey="Robinson L" first="Leanne J." last="Robinson">Leanne J. Robinson</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Walter & Eliza Hall Institute</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a6"><institution>Papua New Guinea Institute of Medical Research</institution>
, Madang,<country>Papua New Guinea</country>
</nlm:aff>
<country xml:lang="fr">Papouasie-Nouvelle-Guinée</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ome Kaius, Maria" sort="Ome Kaius, Maria" uniqKey="Ome Kaius M" first="Maria" last="Ome-Kaius">Maria Ome-Kaius</name>
<affiliation wicri:level="1"><nlm:aff id="a6"><institution>Papua New Guinea Institute of Medical Research</institution>
, Madang,<country>Papua New Guinea</country>
</nlm:aff>
<country xml:lang="fr">Papouasie-Nouvelle-Guinée</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sattabongkot, Jetsumon" sort="Sattabongkot, Jetsumon" uniqKey="Sattabongkot J" first="Jetsumon" last="Sattabongkot">Jetsumon Sattabongkot</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>Faculty of Tropical Medicine, Mahidol University</institution>
, Bangkok,<country>Thailand</country>
</nlm:aff>
<country xml:lang="fr">Thaïlande</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Felger, Ingrid" sort="Felger, Ingrid" uniqKey="Felger I" first="Ingrid" last="Felger">Ingrid Felger</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Swiss Tropical and Public Health Institute</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a5"><institution>University of Basel</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mueller, Ivo" sort="Mueller, Ivo" uniqKey="Mueller I" first="Ivo" last="Mueller">Ivo Mueller</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Walter & Eliza Hall Institute</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Medical Biology, University of Melbourne</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a7"><institution>Institut Pasteur</institution>
, Paris,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27982132</idno>
<idno type="pmc">5159915</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159915</idno>
<idno type="RBID">PMC:5159915</idno>
<idno type="doi">10.1038/srep39183</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000858</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000858</idno>
<idno type="wicri:Area/Pmc/Curation">000857</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000857</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000612</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000612</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">001484</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001484</idno>
<idno type="wicri:Area/PubMed/Curation">001462</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001462</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001462</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001462</idno>
<idno type="wicri:Area/Ncbi/Merge">004041</idno>
<idno type="wicri:Area/Ncbi/Curation">004041</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">004041</idno>
<idno type="wicri:Area/Main/Merge">001745</idno>
<idno type="wicri:Area/Main/Curation">001750</idno>
<idno type="wicri:Area/Main/Exploration">001750</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)</title>
<author><name sortKey="Koepfli, Cristian" sort="Koepfli, Cristian" uniqKey="Koepfli C" first="Cristian" last="Koepfli">Cristian Koepfli</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Walter & Eliza Hall Institute</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Medical Biology, University of Melbourne</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nguitragool, Wang" sort="Nguitragool, Wang" uniqKey="Nguitragool W" first="Wang" last="Nguitragool">Wang Nguitragool</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>Faculty of Tropical Medicine, Mahidol University</institution>
, Bangkok,<country>Thailand</country>
</nlm:aff>
<country xml:lang="fr">Thaïlande</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hofmann, Natalie E" sort="Hofmann, Natalie E" uniqKey="Hofmann N" first="Natalie E." last="Hofmann">Natalie E. Hofmann</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Swiss Tropical and Public Health Institute</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a5"><institution>University of Basel</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Robinson, Leanne J" sort="Robinson, Leanne J" uniqKey="Robinson L" first="Leanne J." last="Robinson">Leanne J. Robinson</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Walter & Eliza Hall Institute</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a6"><institution>Papua New Guinea Institute of Medical Research</institution>
, Madang,<country>Papua New Guinea</country>
</nlm:aff>
<country xml:lang="fr">Papouasie-Nouvelle-Guinée</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ome Kaius, Maria" sort="Ome Kaius, Maria" uniqKey="Ome Kaius M" first="Maria" last="Ome-Kaius">Maria Ome-Kaius</name>
<affiliation wicri:level="1"><nlm:aff id="a6"><institution>Papua New Guinea Institute of Medical Research</institution>
, Madang,<country>Papua New Guinea</country>
</nlm:aff>
<country xml:lang="fr">Papouasie-Nouvelle-Guinée</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sattabongkot, Jetsumon" sort="Sattabongkot, Jetsumon" uniqKey="Sattabongkot J" first="Jetsumon" last="Sattabongkot">Jetsumon Sattabongkot</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>Faculty of Tropical Medicine, Mahidol University</institution>
, Bangkok,<country>Thailand</country>
</nlm:aff>
<country xml:lang="fr">Thaïlande</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Felger, Ingrid" sort="Felger, Ingrid" uniqKey="Felger I" first="Ingrid" last="Felger">Ingrid Felger</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Swiss Tropical and Public Health Institute</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a5"><institution>University of Basel</institution>
, Basel,<country>Switzerland</country>
</nlm:aff>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mueller, Ivo" sort="Mueller, Ivo" uniqKey="Mueller I" first="Ivo" last="Mueller">Ivo Mueller</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Walter & Eliza Hall Institute</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Medical Biology, University of Melbourne</institution>
, Parkville,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="a7"><institution>Institut Pasteur</institution>
, Paris,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Accurate quantification of parasite density in the human host is essential for understanding the biology and pathology of malaria. Semi-quantitative molecular methods are widely applied, but the need for an external standard curve makes it difficult to compare parasite density estimates across studies. Droplet digital PCR (ddPCR) allows direct quantification without the need for a standard curve. ddPCR was used to diagnose and quantify <italic>P. falciparum</italic>
 and <italic>P. vivax</italic>
 in clinical patients as well as in asymptomatic samples. ddPCR yielded highly reproducible measurements across the range of parasite densities observed in humans, and showed higher sensitivity than qPCR to diagnose <italic>P. falciparum</italic>
, and equal sensitivity for <italic>P. vivax</italic>
. Correspondence in quantification was very high (>0.95) between qPCR and ddPCR. Quantification between technical replicates by ddPCR differed 1.5–1.7-fold, compared to 2.4–6.2-fold by qPCR. ddPCR facilitates parasite quantification for studies where absolute densities are required, and will increase comparability of results reported from different laboratories.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Imwong, M" uniqKey="Imwong M">M. Imwong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hofmann, N" uniqKey="Hofmann N">N. Hofmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zimmerman, P A" uniqKey="Zimmerman P">P. A. Zimmerman</name>
</author>
<author><name sortKey="Howes, R E" uniqKey="Howes R">R. E. Howes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Britton, S" uniqKey="Britton S">S. Britton</name>
</author>
<author><name sortKey="Cheng, Q" uniqKey="Cheng Q">Q. Cheng</name>
</author>
<author><name sortKey="Mccarthy, J S" uniqKey="Mccarthy J">J. S. McCarthy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barber, B E" uniqKey="Barber B">B. E. Barber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rubach, M P" uniqKey="Rubach M">M. P. Rubach</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Koepfli, C" uniqKey="Koepfli C">C. Koepfli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Churcher, T S" uniqKey="Churcher T">T. S. Churcher</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Okell, L C" uniqKey="Okell L">L. C. Okell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cheng, Q" uniqKey="Cheng Q">Q. Cheng</name>
</author>
<author><name sortKey="Cunningham, J" uniqKey="Cunningham J">J. Cunningham</name>
</author>
<author><name sortKey="Gatton, M L" uniqKey="Gatton M">M. L. Gatton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vallejo, A F" uniqKey="Vallejo A">A. F. Vallejo</name>
</author>
<author><name sortKey="Garcia, J" uniqKey="Garcia J">J. Garcia</name>
</author>
<author><name sortKey="Amado Garavito, A B" uniqKey="Amado Garavito A">A. B. Amado-Garavito</name>
</author>
<author><name sortKey="Arevalo Herrera, M" uniqKey="Arevalo Herrera M">M. Arevalo-Herrera</name>
</author>
<author><name sortKey="Herrera, S" uniqKey="Herrera S">S. Herrera</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mangold, K A" uniqKey="Mangold K">K. A. Mangold</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rosanas Urgell, A" uniqKey="Rosanas Urgell A">A. Rosanas-Urgell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kamau, E" uniqKey="Kamau E">E. Kamau</name>
</author>
<author><name sortKey="Alemayehu, S" uniqKey="Alemayehu S">S. Alemayehu</name>
</author>
<author><name sortKey="Feghali, K C" uniqKey="Feghali K">K. C. Feghali</name>
</author>
<author><name sortKey="Saunders, D" uniqKey="Saunders D">D. Saunders</name>
</author>
<author><name sortKey="Ockenhouse, C F" uniqKey="Ockenhouse C">C. F. Ockenhouse</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barnwell, J W" uniqKey="Barnwell J">J. W. Barnwell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Karlen, Y" uniqKey="Karlen Y">Y. Karlen</name>
</author>
<author><name sortKey="Mcnair, A" uniqKey="Mcnair A">A. McNair</name>
</author>
<author><name sortKey="Perseguers, S" uniqKey="Perseguers S">S. Perseguers</name>
</author>
<author><name sortKey="Mazza, C" uniqKey="Mazza C">C. Mazza</name>
</author>
<author><name sortKey="Mermod, N" uniqKey="Mermod N">N. Mermod</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hindson, B J" uniqKey="Hindson B">B. J. Hindson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hindson, C M" uniqKey="Hindson C">C. M. Hindson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yang, R" uniqKey="Yang R">R. Yang</name>
</author>
<author><name sortKey="Paparini, A" uniqKey="Paparini A">A. Paparini</name>
</author>
<author><name sortKey="Monis, P" uniqKey="Monis P">P. Monis</name>
</author>
<author><name sortKey="Ryan, U" uniqKey="Ryan U">U. Ryan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jongthawin, J" uniqKey="Jongthawin J">J. Jongthawin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wilson, M" uniqKey="Wilson M">M. Wilson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hou, Y" uniqKey="Hou Y">Y. Hou</name>
</author>
<author><name sortKey="Zhang, H" uniqKey="Zhang H">H. Zhang</name>
</author>
<author><name sortKey="Miranda, L" uniqKey="Miranda L">L. Miranda</name>
</author>
<author><name sortKey="Lin, S" uniqKey="Lin S">S. Lin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dong, L" uniqKey="Dong L">L. Dong</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Papouasie-Nouvelle-Guinée</li>
<li>Suisse</li>
<li>Thaïlande</li>
</country>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Koepfli, Cristian" sort="Koepfli, Cristian" uniqKey="Koepfli C" first="Cristian" last="Koepfli">Cristian Koepfli</name>
</noRegion>
<name sortKey="Koepfli, Cristian" sort="Koepfli, Cristian" uniqKey="Koepfli C" first="Cristian" last="Koepfli">Cristian Koepfli</name>
<name sortKey="Mueller, Ivo" sort="Mueller, Ivo" uniqKey="Mueller I" first="Ivo" last="Mueller">Ivo Mueller</name>
<name sortKey="Mueller, Ivo" sort="Mueller, Ivo" uniqKey="Mueller I" first="Ivo" last="Mueller">Ivo Mueller</name>
<name sortKey="Robinson, Leanne J" sort="Robinson, Leanne J" uniqKey="Robinson L" first="Leanne J." last="Robinson">Leanne J. Robinson</name>
</country>
<country name="Thaïlande"><noRegion><name sortKey="Nguitragool, Wang" sort="Nguitragool, Wang" uniqKey="Nguitragool W" first="Wang" last="Nguitragool">Wang Nguitragool</name>
</noRegion>
<name sortKey="Sattabongkot, Jetsumon" sort="Sattabongkot, Jetsumon" uniqKey="Sattabongkot J" first="Jetsumon" last="Sattabongkot">Jetsumon Sattabongkot</name>
</country>
<country name="Suisse"><noRegion><name sortKey="Hofmann, Natalie E" sort="Hofmann, Natalie E" uniqKey="Hofmann N" first="Natalie E." last="Hofmann">Natalie E. Hofmann</name>
</noRegion>
<name sortKey="Felger, Ingrid" sort="Felger, Ingrid" uniqKey="Felger I" first="Ingrid" last="Felger">Ingrid Felger</name>
<name sortKey="Felger, Ingrid" sort="Felger, Ingrid" uniqKey="Felger I" first="Ingrid" last="Felger">Ingrid Felger</name>
<name sortKey="Hofmann, Natalie E" sort="Hofmann, Natalie E" uniqKey="Hofmann N" first="Natalie E." last="Hofmann">Natalie E. Hofmann</name>
</country>
<country name="Papouasie-Nouvelle-Guinée"><noRegion><name sortKey="Robinson, Leanne J" sort="Robinson, Leanne J" uniqKey="Robinson L" first="Leanne J." last="Robinson">Leanne J. Robinson</name>
</noRegion>
<name sortKey="Ome Kaius, Maria" sort="Ome Kaius, Maria" uniqKey="Ome Kaius M" first="Maria" last="Ome-Kaius">Maria Ome-Kaius</name>
</country>
<country name="France"><noRegion><name sortKey="Mueller, Ivo" sort="Mueller, Ivo" uniqKey="Mueller I" first="Ivo" last="Mueller">Ivo Mueller</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001750 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001750 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:5159915
   |texte=   Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:27982132" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a AustralieFrV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

Serveur d'exploration sur les relations entre la France et l'Australie

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.